Crosslinking agent bakeng sa merero ea N,N'-Methylenebisacrylamide

N,N' -methylene diacrylamide (MBAm kapa MBAA)ke crosslinking moemeli sebelisoa ka sebopeho sa polymers tse kang polyacrylamide. Mokhoa oa eona oa limolek'hule ke C7H10N2O2, CAS: 110-26-9, thepa: phofo e tšoeu ea kristale, e qhibilihang ka metsing, hape e qhibiliha ka ethanol, acetone le lihlahisoa tse ling tsa tlhaho. Diacrylamide ke motsoako oa polyacrylamide gel (bakeng sa SDS-PAGE) e ka sebelisoang ho biochemistry. Diacrylamide polymerizes kaacrylamidemme e khona ho theha likhokahano lipakeng tsa liketane tsa polyacrylamide, ka hona ho etsa marang-rang a polyacrylamide ho fapana le linear e sa amaneng.polyacrylamideliketane.

Moemedi wa ho tshedisana
Ho k'hemistri le biology, crosslinking ke tlamo e hokahanyang ketane e le 'ngoe ea polymer ho e' ngoe. Lihokelo tsena li ka nka sebopeho sa li-covalent kapa ionic bond, 'me polymer e ka ba ea maiketsetso kapa ea tlhaho (mohlala, protheine).
Ka k'hemistri ea polymer, "crosslinking" hangata e bolela tšebeliso ea crosslinking ho khothaletsa liphetoho linthong tsa 'mele tsa polymer.
Ha "crosslinking" e sebelisoa lefapheng la baeloji, e bua ka tšebeliso ea li-probes ho hokahanya liprotheine ho hlahloba likamano tsa protheine le liprotheine le mekhoa e meng e mecha ea ho hokahanya.
Leha lentsoe lena le sebelisoa ho bolela "ho hokahana ha liketane tsa polymer" lithutong tsa mahlale ka bobeli, boemo ba ho hokahana le boits'oaro ba "crosslinking agent" bo fapana haholo. Joalo ka mahlale ohle, ho na le ho kopana, 'me tlhaloso e latelang ke qalo ea ho utloisisa lintlha tsena.

Polyacrylamidegel electrophoresis
Polyacrylamide gel electrophoresis (PAGE) ke mokhoa o sebelisoang haholo ho biochemistry, forensics, genetics, molecular biology, le biotechnology bakeng sa karohano ea li-macromolecules tsa baeloji (hangata liprotheine kapa li-nucleic acid) tse ipapisitseng le motsamao oa tsona oa electrophoretic. Electrophoretic mobility ke ts'ebetso ea bolelele ba molek'hule, conformation, le tefiso. Polyacrylamide gel electrophoresis ke sesebelisoa se matla sa ho hlahloba lisampole tsa RNA. Ha gel ea polyacrylamide e etsoa denatured ka mor'a electrophoresis, e fana ka tlhahisoleseding mabapi le sebopeho sa sampole ea mofuta oa RNA.

Lisebelisoa tse ling tsa N, N' -methylene diacrylamide
N, N' -methylene diacrylamide e le reagent ea lik'hemik'hale e na le mefuta e mengata ea lisebelisoa, ke mokelikeli oa oli ea oli ea oli, resin e kholo, e thibelang metsi, li-additives tsa konkreite, resin ea nylon e qhibilihang ka joala, motsoako oa metsi oa motsoako oa motsoako oa bohlokoa, hape ke ntho e ntle ea ho monya metsi le ho boloka metsi, e sebelisoang ho etsa resin e matla le ntlafatso ea mobu, E boetse e sebelisetsoa ho nka lifoto, khatiso, ho etsa lipoleiti, jj.